Take 100μL of the induced bacterial solution at 4℃, centrifuge at 8000xg for 2min according to the method described in 2.4, collect the bacterial cells, and wash twice with PBS
According to the method of 2.4, take 100 sL-induced good bacteria liquid at 4 degrees C, 8000xg centrifuged 2min, collect the bacteria, PBS wash 2 times
According to the method described in 2.4, 100 μ l of induced bacterial solution was taken and centrifuged at 4 ℃ for 2 min at 8000 XG. The bacteria were collected and washed twice with PBS<br>