The ultra-filtrate was stored at30 C until analysis. The perfusate samples undergone similar procedure as the blood samples. Tissues sampleswere stored at 80℃ until analysis. Platinum determination wasperformed using an iCAP-Q Inductive Coupled Plasma Mass Spectrometry (ICP-MS)° system by Thermo Scientific after preparing calibration curves with atomic platinum. Fluid samples werediluted before ICP-MS examination while tissues were digestedwith a mixture of nitric acid and oxygen water prior to cisplatindetermination.