The plant materials used in this st

The plant materials used in this study include soybean genotypes Williams 82, Jack, H99(P resistance genotype) and H100(P resistance genotype). To study the genotype difference of phosphorus starvation GmPAP17 expression level. The seeds are surface sterilized and then pFull-length ORF sequences of GmPAP17 (Glyma08g056400,) were amplified from soybean genotype Williams 82 root cDNA with gene-specific primers GmPAP17OE-F and GmPAP17OE-R, and then cloned into the vector PMDC83 using Bam HI and Sal I for the overexpression (OE) construct. BLAST analysis of the mRNA sequence of GmPAP17 gene was carried out on NCBI website. Based on the comparison results, the region of the gene from 115 to 523bp was selected as RNA interference region in this experiment. Using Gateway technology, RNAi vectors was cloned with specific primers gMPAP17RI-F and GMPAP17RT-R, whose restriction sites were attB1 and attB2(3). The sequences of the primers are listed in Table S2.lanted in pots with vermiculite. One week after germination, H99 and H100 seedlings were