The laboratory should use a validated test, appropriate to the purpose of testing, which is important whentesting across different species due to potential variation in sensitivity and specificity. If a private laboratorytests animal samples, close communication and coordination should be maintained with the VeterinaryAuthority. If real-time RT-PCR is used for detection and borderline PCR cycle threshold (Ct) values are obtained,further validation and confirmatory testing may be required.The actions to be taken on positive or negative findings should be carefully planned for before samplingand testing of animals for SARS-CoV-2.