To study the anti-tumor activity of the alcohol extract of lychee leaves. Methods: Four types of tumor cells, including A549 (human lung cancer cells), CNE-2 (human nasopharyngeal carcinoma cells), Hela (human cervical cancer cells), and HepG2 (human liver cancer cells), were cultured in vitro, and the alcohol extraction of lychee leaves was examined by MTT method. The inhibitory effect of the substance on the proliferation of the above 4 kinds of tumor cells. Results: Litchi leaf alcohol extracts had inhibitory effects on the proliferation of 4 kinds of tumor cells, and the inhibition rate increased with the increase of the concentration of alcohol extracts. Among them, the inhibitory effects on CNE-2 cells, Hela cells and HepG2 cells were more prominent. Conclusion: The alcohol extract of lychee leaves has anti-tumor activity in vitro.