Figure 1. Example of medium exchang

Figure 1. Example of medium exchange (A) vs. direct addition of 10X probe (B) for live cell imaging. Medium exchange results in uniform exposure of cells to probe, but floating dead cells may be lost during medium removal, and suspension cells must be pelleted by centrifugation. Direct addition of 10X probe avoids loss of cells, but care must be taken to mix immediately yet gently to avoid high transient probe concentration or disruption of cells by pipetting. Volumes shown are for staining cells in a 96-well plate with 100 uL medium per well; volumes should be scaled proportionally for different well sizes. The final concentration of Annexin V may require optimization for different detection systems.