Although forefront research fields on molecular machineries and nano‐manipulation have been developed, their current achievements stay mostly in basic science and are far from practical usages in daily life. In this short review, interfacial nanoarchitectonics for structural and functional tuning of molecular machines, molecular receptors, and related molecular objects is conceptually overviewed towards possible applications. As their typical functions, regulation methods of molecular recognitions and the other functions in three mode, (i) fixed structure mode, (ii) switching mode, and (iii) tuning mode, are mainly discussed. The exemplified facts reveal great possibilities in molecular tuning at interfaces. The functional molecules immobilized at interfacial media can be manipulated in relation with macroscopic human motions, which would be one of the promising ways to use molecular‐level functions in life‐related activities.