The effects of different fertilization methods on red soil paddy soil were studied, and it was shown that the application of inorganic phosphorus fertilizer was the most beneficial to the growth of bacteria in the soil, and the balanced application of inorganic N, P, and K fertilizers was the most beneficial to the growth of bacteria and actinomycetes in the soil. Bai Zhen et al. (2009) showed that NPK combined with organic fertilizer or straw can significantly increase the number of nitrogen-fixing bacteria, ammoniating bacteria, denitrifying bacteria, nitrifying bacteria, and cellulolytic bacteria in the soil compared with single fertilizer application. Due to the complex and huge number of microorganisms in the soil, 99% of the soil microorganisms cannot be cultivated in the laboratory. With the development of the third generation sequencing technology, more and more high-throughput sequencing technology is used to study the soil microbial community structure
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