•Fluorescent dyes for imaging lysosomes in live cells•Highly specific, no-wash staining of acidic organelles•LysoView™ 405 for the DAPI channel•LysoView™ 488 for the FITC channel•Red fluorescent LysoView™ 540 for the Cy®3 channel•Far-red fluorescent LysoView™ 633 for the Cy®5 channelUnique, UV-activated Light-On LysoView™ 555