Briefly, 100 μl aliquots of CFS were placed in wells (6-mm diameter) bored in cooled Alicyclobacillus agar (Pronadisa) plates (30 ml) previously seeded (105 CFU/ml) with A. acidoterrestris sp1. Plates were incubated at 50 °C to allow growth of the target organism and checked for zones of inhibition after 24-48 h.