、生物质存储罐、泵、真空纤维膜系统、产物存储罐、加热装置、控制系统、温

、生物质存储罐、泵、真空纤维膜系统、产物存储罐、加热装置、控制系统、温度传感器、压力传感器等部件.设备运行过程中,蠕动臂和蠕动球在电机带动下,对酶解罐中的生物质物料施加法向作用力并随即撤销,物料在力的施加与撤销循环过程中,达到周期混合,混合的频率可通过控制系统参数进行合理调整.酶解反应后,酶解液体真空纤维膜系统过滤进入产物存储罐,用于后续发酵.低场核磁分析结果表明,与摇床振荡培养相比,在前24 h内,周期蠕动酶解体系中主要水分的信号峰强度增加了32.1 %,表明周期蠕动有效地促进了初始阶段束缚水的释放.此外,两种体系中的表观粘度有明显差异.如图4[22]所示,在酶解前期,周期蠕动使酶解体系中的表观粘度维持在相对较低的水平,表观粘度降低能显着增加高固酶解聚糖转化率.因此,周期蠕动可有效促进酶解初级水 分释放,短时间内降低体系内的表观粘度,强化毛管水传递,因而促进玉米秸秆高固酶解效率.Chen等[24]提出了高固多相生物反应工程理念, Fu等[25]基于该理念发明了先固相强化酶解解聚、后同步糖化全糖发酵新工艺,成功用于万吨级汽爆秸秆炼制工业化示范工程[26],提高了木质纤维素酶解发酵效率. <br>4结语和展望<br>高固多相生物反应体系中由固体基质引起的“固体效应”、“水束缚效应”及其引起的体系流变学特征改变是高固多相生物反应工程面临的主要基础问题.基于本课题组多年的研究, 从固相基质特性及其对高固多相生物反应体系理化特性的影响入手, 剖析了高固多相生物反应速率的限制因素, 发现驱动固相基质中毛管水传递是提高高固多相生物反应体系效率的关键措施之一, 提出了高固多相生物反应工程的理念, 丰富和推动了生物反应工程学科发展.将高固多相生物反应工程理念用于以生物反应为核心的生物基产品生产有助于实现资源、经济、环境效益有机统一.以生物质为原料的生物基产品能充分发挥废弃物资源的价值, 体系中高固形物含量使底物和产物浓度提高, 提高了其分离过程的经济性.同时高固形物含量意味着较少的用水量, 对解决当前发酵行业高能耗、高水耗, 缓解日益恶化的环境污染具有重要意义.高固多相生物反应有别于低固形物体系的生物反应过程, 因而不能简单地将低固反应体系的反应机制及相关措施用于高固多相生物反应过程, 因而有必要系统研究高固多相生物反应过程机理, 开发更多有效的过程强化手段及配套反应器, 从而推动其大规模工业化应用进程.

、生物质存储罐、泵、真空纤维膜系统、产物存储罐、加热装置、控制系统、温度传感器、压力传感器等部件.设备运行过程中, 蠕动臂和蠕动球在电机带动下, 对酶解罐中的生物质物料施加法向作用力并随即撤销, 物料在力的施加与撤销循环过程中 , 达到周期混合, 混合的频率可通过控制系统参数进行合理调整.酶解反应后, 酶解液体真空纤维膜系统过滤进入产物存储罐, 用于后续发酵.低场核磁分析结果表明, 与摇床振荡培养相比, 在前24 h内, 周期蠕动酶解体系中主要水分的信号峰强度增加了32.1%, 表明周期蠕动有效地促进了初始阶段束缚水的释放.此外, 两种体系中的表观粘度有明显差异.如图4[22]所示, 在酶解前期, 周期蠕动使酶解体系中的表观粘度维持在相对较低的水平, 表观粘度降低能显著增加高固酶解聚糖转化率.因此, 周期蠕动可有效促进酶解初级水分释放, 短时间内降低体系内的表观粘度, 强化毛管水传递, 因而促进玉米秸秆高固酶解效率. Chen等[24]提出了高固多相生物反应工程理念, Fu等[25]基于该理念发明了先固相强化酶解解聚、后同步糖化全糖发酵新工艺, 成功用于万吨级汽爆秸秆炼制工业化示范工程[26], 提高了木质纤维素酶解发酵效率.<br>4 结语和展望<br>高固多相生物反应体系中由固体基质引起的“固体效应”、“水束缚效应”及其引起的体系流变学特征改变是高固多相生物反应工程面临的主要基础问题.基于本课题组多年的研究, 从固相基质特性及其对高固多相生物反应体系理化特性的影响入手, 剖析了高固多相生物反应速率的限制因素, 发现驱动固相基质中毛管水传递是提高高固多相生物反应体系效率的关键措施之一, 提出了高固多相生物反应工程的理念, 丰富和推动了生物反应工程学科发展.将高固多相生物反应工程理念用于以生物反应为核心的生物基产品生产有助于实现资源、经济、环境效益有机统一.以生物质为原料的生物基产品能充分发挥废弃物资源的价值, 体系中高固形物含量使底物和产物浓度提高, 提高了其分离过程的经济性.同时高固形物含量意味着较少的用水量, 对解决当前发酵行业高能耗、高水耗, 缓解日益恶化的环境污染具有重要意义.高固多相生物反应有别于低固形物体系的生物反应过程 , 因而不能简单地将低固反应体系的反应机制及相关措施用于高固多相生物反应过程, 因而有必要系统研究高固多相生物反应过程机理, 开发更多有效的过程强化手段及配套反应器, 从而推动其大规模工业化应用进程.

, biomass storage tank, pump, vacuum fiber membrane system, product storage tank, heating device, control system, temperature sensor, pressure sensor and other components. During the operation of the equipment, the peristaltic arm and peristaltic ball, driven by the motor, exert normal force on the biomass material in the enzymolysis tank and then cancel it. During the application and cancellation of the force, the material achieves periodic mixing, The mixing frequency can be adjusted by controlling the parameters of the system. After the enzymatic hydrolysis, the vacuum fiber membrane system of the enzymatic hydrolysis liquid is filtered into the product storage tank for subsequent fermentation. The results of low field NMR analysis show that the signal peak intensity of the main water in the periodic peristaltic enzymatic hydrolysis system increases by 32.1% in the first 24 hours compared with the shaking table culture, indicating that the periodic peristalsis effectively promotes the initial fermentation In addition, the apparent viscosity of the two systems is significantly different. As shown in Figure 4 [22], in the early stage of enzymolysis, periodic peristalsis keeps the apparent viscosity of the enzymolysis system at a relatively low level, and the reduction of the apparent viscosity can significantly increase the conversion rate of high solid enzymolysis glycan. Therefore, periodic peristalsis can effectively promote the release of primary water in enzymolysis, and reduce the apparent viscosity in the system in a short time Based on the concept of high solid multi-phase biological reaction engineering proposed by Chen et al. [24], Fu et al. [25] invented a new process of first solid phase enhanced enzymatic hydrolysis and then simultaneous saccharification of total sugar fermentation, which was successfully used in 10000 ton steam exploded straw refining industrial demonstration project [26], improving the efficiency of lignocellulose enzymatic hydrolysis and fermentation<br>Conclusion and Prospect<br>The "solid effect", "water binding effect" caused by solid matrix and the change of rheological characteristics of the system in high solid multiphase biological reaction system are the main basic problems faced by high solid multiphase biological reaction engineering. Based on the research of our group for many years, starting from the properties of solid matrix and its influence on the physical and chemical properties of high solid multiphase biological reaction system, the high solid multiphase is analyzed It is found that driving capillary water transfer in solid matrix is one of the key measures to improve the efficiency of high solid multiphase biological reaction system. The concept of high solid multiphase biological reaction engineering is put forward, which enriches and promotes the development of biological reaction engineering. It is helpful to apply the concept of high solid multiphase biological reaction engineering to the production of biological based products with biological reaction as the core At present, resources, economy and environmental benefits are unified organically. Bio based products with biomass as raw material can give full play to the value of waste resources. The high solid content in the system can improve the concentration of substrate and product, and improve the economy of separation process. Meanwhile, high solid content means less water consumption, which can solve the current high energy consumption, high water consumption of fermentation industry, and alleviate the worsening environment Environmental pollution is of great significance. High solid multiphase bioreaction is different from that of low solid system. Therefore, the reaction mechanism and relevant measures of low solid system cannot be simply applied to the high solid multiphase bioreaction process. Therefore, it is necessary to systematically study the mechanism of high solid multiphase bioreaction process, develop more effective process strengthening means and supporting reactors, so as to promote Its large-scale industrial application process<br>