三株肺炎克雷伯菌是从临床分离得到的临床分离株，未经过任何改造，感染风险

三株肺炎克雷伯菌是从临床分离得到的临床分离株，未经过任何改造，感染风险可控，后续主要用于药物筛选研究。该菌株首先接种于固体平板，37°C培养箱培养18-24 h，然后挑取单个克隆，接种于液体培养基，37°C摇床培养18-24 h，再取出保存于含有15%甘油的大豆胰蛋白肉汤培养基中，通过干冰运输至辉源生物科技(上海)有限公司，进行药物筛选研究实验。该菌株在BSL-2级实验室中进行实验，研究人员做好安全防护措施，实验过程中若遇到的误操作问题，实验室按规定程序进行消毒灭菌处理，避免实验室人员受到伤害，或者环境污染。

Three pneumonia Crebbacteria are clinically isolated strains from clinical isolation, without any modification, the risk of infection can be controlled, followed by mainly used in drug screening studies. The strain was first inoculated on a solid plate, cultured 18-24 h in a 37-C incubator, then picked a single clone, inoculated in a liquid medium, 37 oC shaker cultured 18-24 h, and then removed from a soybean trylinator culture medium containing 15% glycerin, transported through dry ice to Huiyuan Biotech (Shanghai) Co., Ltd., Conducted drug screening research experiments. The strain in the BSL-2 laboratory experiment, the researchers do a good job of safety measures, in the course of the experiment encountered in the wrong operation problems, the laboratory in accordance with the prescribed procedures for sterilization treatment, to avoid the laboratory personnel injury, or environmental pollution.

The three Klebsiella pneumoniae strains are clinical strains isolated from the clinical, without any modification, the risk of infection is controllable, and they are mainly used in drug screening research. The strain was first inoculated on a solid plate, cultured in a 37 ° C incubator for 18-24 h, then single clone was selected, inoculated in a liquid medium, and cultured in a 37 ° C shaking table for 18-24 h, then it was taken out and stored in a soybean pancreatin broth medium containing 15% glycerol, and transported to Huiyuan Biotechnology (Shanghai) Co., Ltd. through dry ice for drug screening research. The strain was tested in the BSL-2 laboratory, and the researchers took safety protection measures. In case of misoperation during the experiment, the laboratory carried out disinfection and sterilization according to the specified procedures to avoid injury to laboratory personnel or environmental pollution.<br>