（1）配置高尔基溶液，将1g的重铬酸钾溶于20ml双蒸水中配置成5%重

(1) Prepare Golgi solution, dissolve 1g of potassium dichromate in 20ml of double distilled water to make 5% potassium dichromate solution, then dissolve 1g of mercuric chloride in 20ml of double distilled water to make 5% chloride Mercury solution, dissolving 0.8g of potassium chromate in 16ml of double-distilled water to form a 5% potassium chromate solution, then mixing 20ml of 5% potassium dichromate solution with 5% mercuric chloride solution, and finally mixing 16ml Potassium chromate solution mixed with 40ml of these solutions evenly, and stored in the dark for more than 5 days before use. (2) Organization preparation. The brains of the mice were removed and placed in the prepared Golgi solution, stored at room temperature in the dark for 14 days, and dehydrated with 75%, 95%, and absolute ethanol in turn. (3) Prepare vibrating slices, hippocampal slices with a thickness of 120 μm, and place them in a 6-well plate. Soak brain slices in 16% ammonia solution for 30 minutes, rinse twice in double distilled water, 2 minutes each time; soak in 1% sodium thiosulfate solution for 7 minutes, rinse twice with double distilled water, 5 minutes each time; Dehydrate in 75%, 95%, and 100% ethanol for 10 min; clear in xylene for 30 s, and seal with neutral gum.

(1) Prepare Gorky solution, dissolve 1g of potassium dichromate in 20ml of double distilled water to prepare a 5% potassium dichromate solution, then dissolve 1g of mercuric chloride in 20ml of double distilled water to prepare a 5% mercuric chloride solution, dissolve 0.8g of potassium chromate in 16ml of double distilled water to prepare a 5% potassium chromate solution, then mix 20ml of 5% potassium dichromate solution and 5% mercuric chloride solution, and finally evenly mix 16ml of potassium chromate solution with 40ml of these solutions, Store in dark for at least 5 days. (2) Organizational preparation. Mouse brains were taken and placed in a prepared Golgi solution, stored at room temperature in dark for 14 days, and dehydrated with 75%, 95%, and anhydrous ethanol in sequence. (3) Prepare for vibration slicing, with a thickness of 120 for hippocampal slices μ m. Place in a 6-hole plate. Soak the brain slices in 16% ammonia solution for 30 minutes and rinse twice in double distilled water for 2 minutes per time; Soak in 1% sodium thiosulfate solution for 7 minutes, rinse twice with double distilled water for 5 minutes per time; Dehydrate in 75%, 95%, and 100% ethanol for 10 minutes in sequence; Xylene transparent for 30 seconds, neutral gum seal.

(1) preparing Gorky solution, dissolving 1g of potassium dichromate in 20ml of double distilled water to prepare 5% potassium dichromate solution, then dissolving 1g of mercuric chloride in 20ml of double distilled water to prepare 5% mercuric chloride solution, dissolving 0.8g of potassium chromate in 16ml of double distilled water to prepare 5% potassium chromate solution, then mixing 20ml of 5% potassium dichromate solution and 5% mercuric chloride solution, and finally mixing 16. (2) organizational preparation. The brains of mice were taken and placed in the prepared Gorky solution, which was stored at room temperature in the dark for 14 days, and then dehydrated with 75%, 95% and anhydrous ethanol in turn. (3) Prepare for vibration sectioning. The thickness of hippocampal slices is 120 μm, and put them in a 6-well plate. Soak the brain slices in 16% ammonia water solution for 30min, and rinse them in double distilled water for 2min/ time; Soak in 1% sodium thiosulfate solution for 7min, rinse with double distilled water twice, 5 min/ time; Dehydrating in 75%, 95% and 100% ethanol for 10min；; Xylene transparent for 30 s, sealed with neutral gum.