2.2 Extraction of Cinnamon Zeylanic

2.2 Extraction of Cinnamon Zeylanicum barkCinnamon zeylanicum bark was washed with water and ace￾tone to remove impurities and was dried before using for fur￾ther experiments. Different solvents, ethanol, water, andDMSO were subjected to the extraction process. Cinnamonzeylanicum bark pieces (1 g) were dipped into each solvent(50 mL) for 6 h under stirring. Different composition of barkand solvents were investigated. The extraction in aqueousmedium was carried out at 80 °C, while extraction in ethanoland DMSO were carried out at ambient temperature and werefiltered and stored under refrigeration. Subsequently,Cinnamon zeylanicum bark extracts were used for the synthe￾sis of AgNPs.2.3 Estimation of total polyphenol contentThe total polyphenolic content of Cinnamon zeylanicum barkextract was estimated using standard Folin-Ciocalteu’s color￾imetric method [29]. Tannic acid was used as a reference re￾agent for the estimation of total phenol content in differentextracts. One milliliter of Cinnamon zeylanicum bark extractin different solvent was mixed with 0.5 mL of Folin￾Ciocalteu’s reagent and diluted with 5 mL of water. After5 min of mixing, 1.5 mL of 20% Na2CO3 was added forneutralization and the volume was made up to 10 mL withwater. This solution was incubated for 120 min at ambienttemperature after which an intense blue color was observed.The data is expressed in milligrams of tannic acid equivalence(TAE). All the experiments were performed in triplicate mea￾surements and values were presented with standard deviation.