HRS–WASH axis governs actin-mediate

HRS–WASH axis governs actin-mediated endosomal recycling and cell invasion HeLa cells were treated with siRNA over 120 h before fixation in 4% PFA/PBS and labeling with antibodies targeting EEA1 and 647-phalloidin. Quantification of sum intensity of actin on endosome. Maximum-projection images (>30 cells total). n = 3; error bars indicate SEM. **, P < 0.01; ***, P < 0.001. Statistical analysis, one-way ANOVA, Dunnett’s post hoc test. Bars: (main images) 10 µm; (insets) 2 µm. MRG, merge.