2.4. 1 and 1d Effectively Attenuate H2O2-Induced Cell Apoptosis 1 and 1d were also examined the ability to protect against H2O2-triggered cell apoptosis using the Hoechst staining assay (400×). Apoptosis is morphologically characterized by nuclear condensation and segregation. As illustrated in Figure 3, cells exposed to 600 μM H2O2 demonstrated cracking, whereas cells cultured only with DMSO were integral. After pretreatment with 1 or 1d at 10 μM, PC12 cells were better protected compared to TBHQ, which underscores the marked antioxidant ability of 1 and 1d.