（3） 根据设计方案搭建实验平台，并将基于图像技术的微流控检测技术应用

(3) Build an experimental platform according to the design plan, and apply the microfluidic detection technology based on image technology to the detection of nitrite nitrogen concentration for basic experiments. The relationship between the liquid layer thickness, temperature, reagent reaction and color development time, minimum detection limit and other parameters of the chip reaction tank was investigated, and a standard curve for the detection of nitrite nitrogen concentration was drawn. The results show that temperature will not have much influence on the detection results; the thickness of the liquid layer used for detection should be 4mm; the color development time should be 3min; the standard curve is more sensitive to concentration detection between the concentration dimension of 0.4~1mg/L. The detection range is between 0.4~10mg/L, and the lowest detection limit is 0.4mg/L; the recovery rate of standard addition is 96.9%~104.0%; the volume of sample consumption and developer volume are only 1/10 of the spectrophotometric method , Respectively, are 1/250 and 1/50 of the colorimetric card detection method. The detection method designed in this paper has low cost, short time-consuming, high accuracy, and can meet the detection requirements of microfluidic detection technology.

(3) According to the design scheme, the experimental platform is set up and the microflow control detection technology based on image technology is applied to the basic experiment in nitrite nitrogen concentration detection. The relationship between the thickness, temperature, reagent reaction and color-showing time, minimum detection limit and other parameters of the liquid layer of the chip reaction pool was examined, and the standard curve of nitrite nitrogen concentration detection was drawn. The results show that the temperature will not have much effect on the test results, and the thickness of the liquid layer used for testing should be 4mm. The color-playing time should be 3min; The standard curve is sensitive to concentration detection between 0.4 and 1 mg/L, with a detection range of 0.4 to 10 mg/L and a minimum detection limit of 0.4 mg/L; 6.9% to 104.0%, sample consumption volume and color agent volume are only 1/10 of the dlight method, respectively, 1/250 and 1/50 of the color card detection method. The detection method designed in this paper is low cost, short time and high accuracy, which can meet the detection needs of microflow control detection technology.

(3) The experimental platform was built according to the design scheme, and the microfluidic detection technology based on image technology was applied to nitrite nitrogen concentration detection for basic experiments. The relationship among the parameters such as liquid layer thickness, temperature, reagent reaction and color development time, and the minimum detection limit was investigated, and the standard curve of nitrite nitrogen concentration detection was drawn. The results show that the temperature has little effect on the detection results, the thickness of liquid layer used for detection should be 4mm, the color developing time should be 3min, and the detection time should be 4mm; The standard curve is sensitive in the concentration dimension of 0.4 ~ 1mg / L, the detection range is 0.4 ~ 10mg / L, the minimum detection limit is 0.4mg/l; the recovery rate is 96.9% ~ 104.0%; the sample consumption volume and developer volume are only 1 / 10 of spectrophotometry, which are 1 / 250 and 1 / 50 of colorimetric card method, respectively. The detection method designed in this paper has the advantages of low cost, short time consuming and high accuracy, which can meet the detection requirements of microfluidic detection technology.<br>