that amino acid residues in the ant

that amino acid residues in the antibody framework significantly affects antibody affinity, specificity and stability. People in the art of the skill in this field knows Alteration of antibody framework while preserve the antibody function and features was not “obvious or “常规改造” with a reasonable expectation of success”. Antibody humanization is particularly an example in which CDRs of rodent antibodies are grafted to a suitable human framework while maintaining the original activity. Today, there is no well-identified strategy to create recombinant humanized antibody V-domains that preserve the antigen-binding characteristics of the parental antibody associated with high affinity, stability and solubility. The big challenging for this work is how to identify/screen for suitable human framework or generate mutations at key residues. As a result, many humanized antibodies in which only framework is changed have generated patents in the world (ref: Patents: US7732578B2 - Super humanized antibodies 2002).