Microbiology has been largely developed thanks to the discovery and optimization of culture media. The first liquid artificial culture mediumwas created by Louis Pasteur in 1860. Previously, bacterial growth on daily materials such as some foods had been observed. Theseobservations highlighted the importance of the bacteria’s natural environment and their nutritional needs in the development of culturemedia for their isolation. A culture medium is essentially composed of basic elements (water, nutrients), to which must be addeddifferent growth factors that will be specific to each bacterium and necessary for their growth.The evolution of bacterial culture through the media used for their culture began with the development of the first solid culture medium byKoch, allowing not only the production of bacterial colonies, but also the possibility of purifying a bacterial clone. The main gelling agent usedin solid culture media is agar. However, some limits have been observed in the use of agar because of some extremely oxygen-sensitivebacteria that do not grow on agar media, and other alternatives were proposed and tested. Then, the discovery of antimicrobial agentsand their specific targets prompted the emergence of selective media. These inhibiting agents make it possible to eliminate undesirablebacteria from the microbiota and select the bacteria desired. Thanks to a better knowledge of the bacterial environment, it will bepossible to develop new culture media and new culture conditions, better adapted to certain fastidious bacteria that are difficult to isolate.© 2019 The Authors. Published by Elsevier Ltd.
Microbiology has been largely developed thanks to the discovery and optimization of culture media. The first liquid artificial culture mediumwas created by Louis Pasteur in 1860. Previously, bacterial growth on daily materials such as some foods had been observed. Theseobservations highlighted the importance of the bacteria’s natural environment and their nutritional needs in the development of culturemedia for their isolation. A culture medium is essentially composed of basic elements (water, nutrients), to which must be addeddifferent growth factors that will be specific to each bacterium and necessary for their growth.The evolution of bacterial culture through the media used for their culture began with the development of the first solid culture medium byKoch, allowing not only the production of bacterial colonies, but also the possibility of purifying a bacterial clone. The main gelling agent usedin solid culture media is agar. However, some limits have been observed in the use of agar because of some extremely oxygen-sensitivebacteria that do not grow on agar media, and other alternatives were proposed and tested. Then, the discovery of antimicrobial agentsand their specific targets prompted the emergence of selective media. These inhibiting agents make it possible to eliminate undesirablebacteria from the microbiota and select the bacteria desired. Thanks to a better knowledge of the bacterial environment, it will bepossible to develop new culture media and new culture conditions, better adapted to certain fastidious bacteria that are difficult to isolate.© 2019 The Authors. Published by Elsevier Ltd.<br>
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