1.Run a gel according to your standard protocol and stain it with a fluorescent stain, such as GelGreen™ Nucleic Acid Gel Stain.
2.Place the provided glass plate into the gel staging area. Place the stained gel on the glass plate.
3.Turn on the Gel-Bright™ device and turn the dial until the desired light intensity is attained.
4.View the gel through the amber filter. For best results do this in a dimly lit, or dark room. If desired, the amber filter may be positioned close to the gel to obtain a better image (see Figure 1C). To adjust the position of the filter, push the Filter Adjustment Knob (Figure 2) up at a 45º angle, and then lower the filter.
5.The gel can be photographed with a regular camera.
6.When excising gel slices, the amber filter can be angled up for better access to the gel (see Figure 1B), or alternatively, the device may be turned around to gain access from the back of the device.
Note: When excising gel slices ALWAYS position the glass plate under the gel, as sharp blades will scratch the lower surface of the gel staging area.