Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis (pink), or with DMSO as a negative control (blue) for the times indicated, then stained for 15 minutes at room temperature with NucView® 530 Caspase-3 Substrate (FL1-H, x-axis) and CF®640R Annexin V (FL4-H, y-axis) in cell culture medium prior to analysis using a BD LSRII flow cytometer. See pp. 4-5 for more information in NucView® Substrates.