in the development of lung cancer,

in the development of lung cancer, due to the lack of effective early screening and diagnosis of tumor molecular markers, most of the clinically diagnosed lung cancer patients are advanced, resulting in patients missing the best treatment opportunities, so that the survival rate is significantly reduced. a large number of studies have shown that there are some miRNA, in the blood of lung cancer patients that are expected to screen out early lung cancer biomarker molecules by high-throughput sequencing for early diagnosis and treatment of lung cancer. miRNA analysis of different expression levels helps us understand the pathogenesis of lung cancer and clarify the role of miRNA in lung cancer the whole genome miRNA expression levels were compared between lung cancer tissues and normal lung tissue samples according to our group's previous high-throughput sequencing data, and verified in TCGA database to determine the miR-20b as the study object. this chapter we investigated the expression of miR-20b in plasma and lung cancer cell lines in NSCLC patients.