8．基于霸王转录组克隆ZxCNGC2基因，其编码697个氨基酸，编码的

8. Transcription based King genomic clone ZxCNGC2 gene, which encodes 697 amino acids, the proteins encoded by Populus trichocarpa (Populus trichocarpa), Populus (Populus euphratica) and castor (Ricinus communis) sequence similarity of 80%, ZxCNGC2 gated Overlord group B subclass ion channel protein family subfamily ⅳ ,, with the peach PpCNGC2, PpCNGC4, PpCNGC8, Arabidopsis AtCNGC4, corn ZmCNGC2, barley HvCNGC4, Lin NnCNGC2 and dates of PdCNGC2 clustered into one branch. Subcellular localization at the plasma membrane. Under different treatment conditions in King ZxCNGC2 roots, stems and leaves were expressed, Na + induced expression of its transcript abundance was higher than Ca2 +, 50 Mm NaCl treatment after expression in roots ZxCNGC2 significantly higher than the stems and leaves, after 0.01 Mm CaCl2 treatment in King ZxCNGC2 roots, stems higher expression. Leaves and roots, the expression level of the highest ZxCNGC2 50 Mm NaCl treatment.

8. Based on the overlord transcription group clone ZxCNGC2 gene, which encodes 697 amino acids, encodes proteins with 80% similarity to populus trichocarpa, populus euphratica and ricinus communis sequences , ZxCNGC2 belongs to The B subclass in the Dominant Gated Ion Channel Protein Family Subspecies IV, and with Peach's PpCNGC2, PpCNGC4, PpCNGC8, Atoma Mustard AtCNGC4, Corn ZmCNGC2, Barley HvCNGC4, Lotus NnCNGC2 and PdCNGC2 of The Sea otter cluster into a branch. Subcells are located on the mass membrane. Under different treatment conditions, ZxCNGC2 is expressed in the dominant root, stem and leaf, the induced expression of The Transcribe abundance of Na is significantly higher than that of Ca2 plus, the expression of ZxCNGC2 in the root after 50 Mm NaCl treatment is significantly higher than that of the stem and leaf, and the Expression of ZxCNGC2 after the treatment of 0.01 Mm CaCl2 is in the overlord root, Stems expressed higher. Of the blades and roots, zxCNGC2 has the highest expression under 50 Mm NaCl processing.

8. Zxcngc2 gene was cloned based on the transcriptome of T.Rex, which encodes 697 amino acids. The sequence similarity of the protein with Populus trichocarpa, Populus euphratica and Ricinus communis is 80%. Zxcngc2 belongs to group B subclass of t.rex-gated-ion-channel protein family Ⅳ, and with ppcngc2, ppcngc4, ppcngc8 of peach and a of Arabidopsis Tcngc4, corn zmcngc2, barley hvcngc4, lotus ncngc2 and jujube pdcngc2 were clustered into one branch. Subcellular localization on plasma membrane. Under different treatment conditions, zxcngc2 was expressed in the root, stem and leaf of Bawang, the induced expression of Na + was significantly higher than that of Ca2 +, the expression of zxcngc2 in the root of 50 mM NaCl treatment was significantly higher than that in the stem and leaf, and the expression of zxcngc2 in the root and stem of Bawang was higher after 0.01 mM NaCl treatment. Among the leaves and roots, the highest expression of zxcngc2 was found under 50 mM NaCl treatment.