3.1V. parahaemolyticus is one of th

3.1V. parahaemolyticus is one of the most important foodborne pathogens that poses a severe threat to food safety, public health, and leads to substantial economic losses worldwide[10]. Several methods have been employed to detect V. parahaemolyticus, including PCR[8,9,11,12]. PCR has been reported to detect various pathogens for decades; it is less time consuming, much simpler, and more economical than traditional culture-based methods. Yu et al.[8] developed a PCR method for detecting the irgB gene to distinguish V.