The use of composite tags fused to a protein of interest to improve protein expression, solubility and purification has gained increasing popularity in protein science. Despite their advantages, fusion tags can introduce a various number of undesired amino acids to the N-terminus and/or to the C-terminus in the protein of interest. To solve this problem, cleavable fusion tags have been developed. The nature of these cleavable tags however limits their usability to either the N- or the C-terminus. Moreover, cleavage of the vast majority of these tags, for example the ones derived from the TEV technology, leaves undesired amino acids that may interfere with protein function.
The use of composite tags fused to a protein of interest to improve protein expression, solubility and purification has gained increasing popularity in protein science. Despite their advantages, fusion tags can introduce a various number of undesired amino acids to the N-terminus and/or to the C-terminus in the protein of interest. To solve this problem, cleavable fusion tags have been developed. The nature of these cleavable tags however limits their usability to either the N- or the C-terminus. Moreover, cleavage of the vast majority of these tags, for example the ones derived from the TEV technology, leaves undesired amino acids that may interfere with protein function.<br>
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