IVUS examination day the blood samples were taken from patients, and separated at a speed of 3000 rev / min for 15 minutes at 4 ℃. The supernatant was transferred to a sterile 2.0 ml Eppendorf tubes, and stored at -80 ℃.
On the day of the IVUS examination, the patient's blood sample was taken and separated for 15 minutes at a speed of 3000 rpm at 4 degrees C. Transfer the supernatant to a sterile 2.0 ml Eppendorf test tube and save at -80 c.
Blood samples were collected on the day of IVUS examination and separated for 15 minutes at 3000 rpm at 4 ℃. The supernatant was transferred to a sterile 2.0 ml Eppendorf tube and stored at - 80 ℃.