from C57BL/6 mice into the mammary

from C57BL/6 mice into the mammary fat pad and monitored tumour growth. We observed that development of obesity significantly increased TNBC growth, leading to tumours having twice the volumes of the ND group after 17 days (Figure 1D). In order to discriminate the impact of obesity-related disorders on TNBC growth from the impact of the HFD itself, we tested the growth of 4T1 TNBC cells derived from Balb/cJRj mice, which are described as an obesity-resistant model upon HFD feeding (Figure 1E).