Average the duplicate readings for

Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density (O.D.).Create a standard curve by reducing the data using computer software capable of generating a log/log curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on a log/log graph. The data may be linearized by plotting thelog of the human ST2 concentrations versus the log of the O.D. on a linear scale, and the best fit line can be determined by regression analysis.If the samples have been diluted, the concentration read from the standard curve must bemultiplied by the dilution factor.