The purpose of College Students' physical health test is to understand and master the health status of college students and the effect feedback and test results of physical exercise reflect the health of college students. A main indicator of students' health status tests the impact of college physical education. From now on, colleges and universities should carry out corresponding physical education reform around students' physical health. This paper analyzes the relationship between the physical health test of college students and the reform of physical education in Colleges and universities, so as to provide scientific basis for the reform of physical education in Colleges and universities.