提取的总RNA 溶于水，加入氯化锂溶液终浓度为2.5M颠倒混匀，-20

The total RNA extracted was dissolved in water, lithium chloride solution was added to a final concentration of 2.5M mix by inversion, -20 ° for 1 hour, centrifuged at 13000rpm at 4 ° C 15 minutes; the supernatant transferred to a clean 1.5 ml EP tube , an equal volume of isopropanol, 0.3 M sodium acetate (pH 5.2), mix by inversion and incubated at 20 degrees for 30 minutes, centrifuged at 4 degrees C 13000rpm 15 minutes. The supernatant was removed, the precipitate was washed with 70% ethanol, evaporated to dryness, water (excluding RNA enzymes) was dissolved.

The total RNA extracted is soluble in water, with a final concentration of 2.5M inverted mixing with lithium chloride solution, 1 hour after -20 degrees incubation, centrifuged at 4 degrees Celsius 13000rpm for 15 minutes; Mix upside down and incubate at -20 degrees for 30 minutes, 4 degrees Celsius 13,000 rpm centrifugal for 15 minutes. Remove the upper clear, wash the precipitation with 70% ethanol, evaporate, and add water (without RNA enzymes) to dissolve.

The extracted total RNA was dissolved in water, and the final concentration of lithium chloride solution was 2.5m. After incubation at - 20 ℃ for 1 hour, it was centrifuged at 13000rpm for 15 minutes. The supernatant was transferred to a clean 1.5ml EP tube, and isopropanol and 0.3m sodium acetate (pH 5.2) of equal volume were added. After incubation at - 20 ℃ for 30 minutes, it was centrifuged at 13000rpm for 15 minutes. Remove the supernatant, wash the precipitate with 70% ethanol, evaporate it, and dissolve it with water (excluding RNA enzyme).<br>