200 μL into the remaining tubes. Use the stock solution to produce a dilutioASSAY PROCEDUREBring all reagents and samples to room temperature before use. It is recommended thatall samples, controls, and standards be assayed in duplicate.1. Prepare all reagents, working standards, and samples as directed in the previous sections.2. Remove excess microplate strips from the plate frame, return them to the foil pouchcontaining the desiccant pack, and reseal.3. Add 50 μL of Assay Diluent RD1-63 to each well.4. Add 50 μL of standard, control, or sample* per well. Cover with the adhesive strip provided.Incubate for 2 hours at room temperature on the benchtop.5. Aspirate each well and wash, repeating the process three times for a total of four washes.Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifolddispenser, or autowasher. Complete removal of liquid at each step is essential to goodperformance. After the last wash, remove any remaining Wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.