NucView® is made by attaching a fluorogenic DNA binding dye to the caspase-3/7 substrate peptide sequence DEVD. Once the substrate is cleaved by caspases in apoptotic cells, it releases the high- affinity DNA dye, which stains the cell nucleus with a bright and stable fluorescent signal (Figure 1). In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® substrates can be used to detect caspase-3/7 activity in cells without inhibiting apoptosis progression.