十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)是用于检测蛋白质

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a method for detecting the effect of protein separation. This method is based on the difference in molecular weight of protein subunits to achieve protein separation. In this experiment, recombinant human follicle stimulating hormone (rhFSH) was used as a sample, and the separation of the gel and protein bands were studied by SDS-PAGE electrophoresis. The gel separation effect of different SDS content is different, and the protein bands detected by different loading volumes are also different. In this experiment, the separation effect of 8% and 13.5% gels was first compared, and it was concluded that the 13.5% gel was suitable for the detection of rhFSH protein. Change the sample loading volume, use the selected 13.5% gel for electrophoresis detection, and compare and analyze the gel map to find that different loading volumes have different effects on the gel band, which is suitable for rhFSH sample loading volume of 10 μL. Finally, the protein activity of rhFSH was studied, and the pH and temperature were tested separately. Different pH values ​​were selected to accelerate the reaction in a 30 ° C incubator. When the pH value is 7, rhFSH protein activity is relatively stable.

十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)是用于检测蛋白质分离效果的方法，该方法是根据蛋白质亚基分子量的差异实现蛋白质分离。本实验以重组人促卵泡细胞激素（rhFSH）为样品，采用SDS-PAGE电泳法对凝胶的分离和蛋白条带进行研究。不同的SDS含量的凝胶分离效果是不同的，不同的上样体积检测出的蛋白条带也是不同的。本实验首先对比8%和13.5%的凝胶的分离效果，得出含量为13.5%的凝胶适合检测rhFSH蛋白。改变样品上样体积，利用选出的13.5%凝胶进行电泳检测，对比分析凝胶图得出不同上样体积对凝胶条带产生不同的影响，适合rhFSH样品上样体积为10μL。最终对样品rhFSH进行蛋白活性研究，分别从pH值和温度进行测试，选择不同pH值进行30℃恒温培养箱中加速反应，当pH值为7时，rhFSH蛋白活性较稳定。

Twelve sodium alkylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method used to detect protein separation effect. The method is based on the difference of protein subunit molecular weight to achieve protein separation. In this study, recombinant human follicle stimulating hormone (rhFSH) was taken as sample and SDS-PAGE gel electrophoresis was used to study gel separation and protein strip. The gel separation effect of different SDS content is different, and the protein bands detected by different sample volume are different. This experiment first compares the separation effect of 8% and 13.5% gel, and concludes that the gel with 13.5% content is suitable for the detection of rhFSH protein. The sample size was changed, and the 13.5% gel was selected for electrophoresis. Compared with the gel graph, the different volume of the gel had different effects on the gel strip, which was suitable for rhFSH sample with a volume of 10 L. Finally, the protein activity of the sample rhFSH was studied, and the pH value and temperature were tested respectively. Different pH values were selected to accelerate the reaction in the constant temperature incubator at 30 ℃. When the pH value was 7, the protein activity of rhFSH was stable.<br>