Example 6一Comparative Analysis[0125

Example 6一Comparative Analysis[0125] The present example demonstrates the enhanced purity of the glargine analog productaccording to Example 4. FIG. 3 depicts an analytical HPLC overlay of LANTUS) (A) and theglargine analog (B). The glargine analog demonstrates increased purity with respect to relatedsubstances and multimeric species over LANTUS.. In the related substance region, the glarineanalog shows noticeably lower levels of contaminants in both the related substance region andthe multimeric region. Most notably, the number of multimeric species is much lower in theglargine analog. Overall purity for the LANTUS. material (A) in the current profile was 98.8%,while the glargine product produced by the herein described method (B) was 99.6%.