When retinal whole mounts from unin

When retinal whole mounts from uninjected eyes of animals with an intravitreal injection of PBS (Fig. 44.1a) or NMDA (Fig. 44.1b) in their contralateral eye were stained for the microglia marker Iba-1, ramified microglia cells showing their typi-cal long branching processes and a small cellular body were readily observed in the ganglion cell layer and both plexiform layers of the retina (Fig. 44.1a, b). An identical shape of microglia cells was observed in retinal whole mounts of untreat-ed animals (not shown). In contrast 3 h after an intravitreal injection of PBS or NMDA, a complete different phenotype of the retinal microglia in the inner retina was observed throughout the retinal whole mount of injected eyes.