During the development of chestnut fruit, the size of chestnut bud and ovary increased with the increase of time, showing an "S" curve. And the fruit development period is generally from July to August, and the mature period is in September. After the original embryo, it is a globular embryo at 28-35d after the full blooming period, a heart-shaped embryo at 35-40d after the full blooming period, and a torpedo-shaped embryo at 40-42d after the full blooming period. , 42-49d after the full flowering stage, the embryo is a cotyledon-shaped embryo, 104d after the full flowering stage, the embryo matures and forms a seed kernel. In the process of embryo development, endosperm cells also undergo cellularization, differentiation, and death, which indirectly proves that there are no endosperm cells in the embryo during the mature embryo. With the development of the embryo, the nutrient protein starch, protein, and polysaccharides in the embryo show an increasing trend. In the nutrient positioning of the embryo development process, starch is mainly located in the outer integument cells, polysaccharides are mainly in the inner integument cells, and the inner and outer beads. There are almost no protein particles in the quilt. With the development of the seed kernel, the starch grains in the cotyledons gradually increased in volume and density, and the embryo development to 51d was the early stage of starch formation, the period from 58-94d was the critical period for the formation of chestnut starch; after 94, it entered the late stage of starch granule formation.
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