4) After Autosampler has finished calibration, it will pause and wait for acceptance of calibration coefficient. To accept coefficient hit Escape and enter Yes to the "Save coefficient" prompt.Sample Analysis1) Go back to Main Menu, select Analysis.2) In Analysis Menu screen, select Sample File that was created in step 7.3) Hit F10 to restart the autosampler for sample analysis. Enter Yes to the “Select racks” prompt. Make sure the rack codes match the type of racks you are using. Code 24 has 14 positions and uses the 50 ml centrifuge tubes. Code 22 has 44 positions and uses the 15 ml centrifuge tubes. Type in the correct codes then hit F10 to begin analysis. 4) Hit any key to begin autosampling. The three standards will be reanalyzed as samples and then the remaining sample solutions in the file will be analyzed.