For CLSM investigation, 100µl of ex

For CLSM investigation, 100µl of exosomes were mixed with 5µl Dil for 20 min at room temperature. After that, 2 ml PBS was added to bind excess dye. Then labeled exosomes were washed in PBS at 100,000 g for 1 hour. The labeled exosomes were incubated with Ti disks and Ti-pepetides disks for 1 h at 4°C and then rinsed thrice with sterile PBS. Exosomes through chimeric peptides specific binding to Ti surfaces was visualized using CLSM. DFLs were incubated with DiI labeled exosomes in an incubator with 5% CO2 in the dark for 12h and 24h. A 1:1000 DAPI nuclear staining solution was prepared with PBS. The cells were placed on ice, washed three times with PBS and then incubated in the dark for 30 min with the diluted DAPI stain. Four different fields of view in each group were randomly imaged with CLSM.