The initial spheroid structure is produced by pipetting a single-cell suspension of 1000 tumor cells in a 20-μl droplet on the inside of the lid of a, for example, 48-well cell culture plate (Greiner Cellstar, BioExpress, Kaysville, UT). Gravity-enforced self-aggregation is facilitated by inverting the lid and placing it on its receptacle (i.e., the 48-well plate). The culture medium and incubation conditions do not need to be adjusted and thus can stay as preferred. After 72 h, the lids are set inside up and the addition of 2000 EC in a volume of 5 μl is added to the existing culture. The lid is then re-inverted, and the hanging drops can be incubated and monitored for growth, cell type incorporation characteristics, and other aspects for an additional 14 days depending on the cell types used (Fig. 8)
The initial spheroid structure is produced by pipetting a single-cell suspension of 1000 <br>tumor cells in a 20-μl droplet on the inside of the lid of a, for example, 48-well cell culture <br>plate (Greiner Cellstar, BioExpress, Kaysville, UT). Gravity-enforced self-aggregation is <br>facilitated by inverting the lid and placing it on its receptacle (i.e., the 48-well plate). The <br>culture medium and incubation conditions do not need to be adjusted and thus can stay as <br>preferred. After 72 h, the lids are set inside up and the addition of 2000 EC in a volume of 5 <br>μl is added to the existing culture. The lid is then re-inverted, and the hanging drops can be <br>incubated and monitored for growth, cell type incorporation characteristics, and other <br>aspects for an additional 14 days depending on the cell types used (Fig. 8)
正在翻译中..