The bacteria were added to 10 mL of 0.5% (w/v) chitosan solution (pH=5.5), 37°C, 800 rpm for 60 minutes, 8000xg, centrifugation for 5 minutes to remove the bacteria and unreacted chitosan.
Add the bacteria to a 10 mL 0.5% (w/v) chitosacsolution solution (pH-5.5), react 60min at 37 degrees C, 800 rpm, and remove the bacteria and unreactive shell polysaccharides by 8000xg, centrifugal 5min.
The bacteria were added into 10 ml 0.5% (w / V) chitosan solution (pH = 5.5), reacted at 37 ℃, 800 rpm for 60 min, and centrifuged for 5 min through 8000 XG to remove the bacteria and unreacted chitosan.<br>