本研究以积盐型盐生植物霸王为材料，首先开展盐生植物霸王和甜土植物拟南芥

In this study, the product salt type Halophytes King of material, is first carried out Halophytes King and sweet soil Arabidopsis Comparative vacuolar membrane Na + / H + antiporter bioinformatics system; carry Halophytes King and proposed based on the above results Arabidopsis thaliana vacuolar membrane Na + / H + antiporter Na +, K + ion transport characteristics analysis; while carrying out King ZxNHX1-RNAi to the wild-type plant RNA-seq sequenced, and using WGCNA analyzing the response difference salt treatment of expression of a gene; obtained based on the above analysis filters King key genes in response to salt treatment ZxCNGC2, ZxCNGC4, the cloned gene ZxCNGC2, ZxCNGC4 carry bioinformatics and expression pattern analysis. Achieved the following main results:

In this study, the salt-based salt-growing plant-breeding plant king as the material, first of all, to carry out the salt-growing plant-based plant-based plant and sweet soil plant amoeba liquid bubble membrane Na / H and reverse transport protein bioinformatics system comparison; Characteristic analysis; at the same time, the dominant ZxNHX1-RNAi and wild plant RNA-seq sequencing, and the use of WGCNA analysis response salt processing differential expression genes; ZxCNGC4 conducts bioinformatics and expression pattern analysis. The following key results were achieved:

Based on the above results, the transport characteristics of Na + / H + antiporter in vacuole membrane of halophyte overlord and Arabidopsis thaliana were analyzed. Meanwhile, the RNA SEQ of zxnhx1 RNAi and wild type plants were measured The key genes zxcngc2, zxcngc4, the cloned genes zxcngc2, zxcngc4 were screened based on the above analysis. The main results are as follows: