将融化的P19细胞移入含生长培养基的离心管中，800g离心5分钟，弃上

Transfer the thawed P19 cells into a centrifuge tube containing growth medium, centrifuge at 800g for 5 minutes, and discard the supernatant. Add 10ml of growth medium to the centrifuge tube again, suspend the cells, centrifuge at 800g for 5 minutes, discard the supernatant, add 4ml of growth medium, and resuspend the cells

The melted P19 cells were transferred into a centrifuge tube containing growth medium, centrifuged at 800g for 5 minutes, and the supernatant was discarded. Add 10ml growth medium again in the centrifuge tube, suspend the cells, centrifuge 800g for 5 minutes, discard the supernatant, add 4ml growth medium and resuspend the cells<br>

Transfer melted P19 cells into a centrifuge tube containing growth medium, centrifuge at 800g for 5min, and discard supernatant. Add 10ml of growth medium into the centrifuge tube again, suspend the cells, centrifuge at 800g for 5min, discard the supernatant, add 4ml of growth medium, and resuspend the cells