3) On the last line of Setup Program Menu, select Yes for Use Autosampler, this will prompt Rinse Time on the menu screen. Input 60* seconds for rinse time and hit enter. (* Adjust rinse time if necessary to ensure thorough rinsing between samples)4) Hit Escape key and wait for Autosampler to initialize.Create Sample File1) Hit Escape key to go back to Main Menu.2) Select your method and check that the flush time in your method parameters is set to 50 seconds (adjust time if necessary to ensure that the instrument does not start taking readings before your sample gets to the plasma).3) In Main Menu, select Analysis.4) In Analysis Menu, select Sample File and Hit Enter.5) Enter Sample File Name. It will create a new file if Sample File Name is new. If Sample File Name already existed, it will indicate so.6) Input sample name for the first sample. 7) Select elements of interest by highlighting the element symbols on the periodic table. Hit F3 to record.8) In the same manner, input sample name for the other samples.Note: Be sure to put low standard as the first sample, mid standard as the second sample and high standard as the third sample. It is recommended to place a mid standard between the samples periodically (every 10 samples) to check for drift.(Caution: Do not use STDLOW, STDMID and STDHIGH as sample names for the three standard solutions. These names are reserved for special function).