The advantages of UPLC systems over

The advantages of UPLC systems over HPLC are obvious. The seprtion mechanism is stll thesarde, while speed, sensitivity and resolution are improved. This all supports easier method transferfrom HPLC to UPLC and its revalidation.The main advantage was particularly a significant reduction of analysis time, which meantalso reduction in solvent consumption. Our experiments showed analysis time was 4.7-6.9 foldshortened, while solvent consumption decreased 5.6-8.5 times. From this point of view, UPLC ismore convenient for complex analytical determination of pharmaceutical preparations in terms ofanalysis duration, solvent consumption and consequently analysis cost. Moreover, the time spenton new method optimization is saved. The time needed for method development experiments, forcolumn equilibration or re equilibration while using gradient elution and for method validation isnuch shorter.

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结果 (英语) 1: [复制]

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The advantages of UPLC systems over HPLC are obvious. The seprtion mechanism is stll thesarde, while speed, sensitivity and resolution are improved. This all supports easier method transferfrom HPLC to UPLC and its revalidation. The main advantage was particularly a significant reduction of analysis time, which meantalso reduction in solvent consumption. Our experiments showed analysis time was 4.7-6.9 foldshortened, while solvent consumption decreased 5.6-8.5 times. From this point of view, UPLC ismore convenient for complex analytical determination of pharmaceutical preparations in terms ofanalysis duration, solvent consumption and consequently analysis cost. Moreover, the time spenton new method optimization is saved. The time needed for method development experiments, forcolumn equilibration or re equilibration while using gradient elution and for method validation isnuch shorter.

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结果 (英语) 2:[复制]

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The advantages of UPLC systems over HPLC are obvious. The seprtion mechanism is stll thesarde, while speed, sensitivity and resolution are improved. This all supports easier method transferfrom HPLC to UPLC and its revalidation. The main advantage was particularly a significant reduction of analysis time, which meantalso reduction in solvent consumption. Our experiments showed analysis time was 4.7-6.9 foldshortened, while solvent consumption decreased 5.6-8.5 times. From this point of view, UPLC ismore convenient for complex analytical determination of pharmaceutical preparations in terms ofanalysis duration, solvent consumption and consequently analysis cost. Moreover, the time spenton new method optimization is saved. The time needed for method development experiments, forcolumn equilibration or re equilibration while using gradient elution and for method validation isnuch shorter.

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结果 (英语) 3:[复制]

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与高效液相色谱相比，UPLC系统的优势显而易见。该分离机构结构简单，速度快，灵敏度高，分辨率高。这一切都支持更容易的方法从高效液相色谱到UPLC及其再验证。 其主要优点是大大缩短了分析时间，这意味着溶剂消耗也减少了。实验表明，分析时间缩短了4.7-6.9倍，溶剂消耗减少了5.6-8.5倍。从这一点来看，UPLC在分析时间、溶剂消耗和分析成本方面更方便用于药物制剂的复杂分析测定。同时，节省了新方法优化的时间。方法开发实验、柱平衡或使用梯度洗脱时重新平衡以及方法验证所需的时间更短。

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