Intensive actin filaments assembly

Intensive actin filaments assembly is associated with ICMS and arrest of CTCsAs cytoskeleton is critical for global architecture and shape of the cells, we next labeled the F‐actin in living CTCs by pCMV LifeAct‐GFP to investigate dynamics of F‐actin filaments in the ICMS19 (Fig. 3a). First, to prove the LifeAct is a qualified living tracer for the F‐actin filaments, after transfected with LifeAct‐GFP, tumor cells were either treated with lysophosphatidic acid (specifically activates Rho subfamily and induce the F‐actin filaments formation) (LPA, 10 μM)20 or Cytochalasin B (efficiently depolymerize the F‐actin) (CB, 1 μM). Eight hours after drugs treatment, confocal imaging was applied and the results have confirmed that the LifeAct‐GFP actually worked as expected (Fig. 3b).