Bacterial wilt is a bacterial soil-borne vascular bundle disease caused by Ralstonia solanacearum. This strain has strong habitability and can survive in soil and non-host plants for a long time. At the same time, Ralstonia solanacearum is a compound species with genetic diversity. It has obvious physiological differentiation and high variability in the process of co-evolution with its host in different geographical origins, so it has always been a difficult problem to control Ralstonia solanacearum. At present, the methods to control bacterial wilt are mostly the utilization of resistant varieties and chemical control, but the resistant varieties will gradually weaken or even lose with the disease resistance from generation to generation, and chemical control will also bring serious damage to the environment and endanger human health. Biological control is a means to control plant diseases by using organisms and their metabolites, among which microbial control has the characteristics of no drug resistance and is friendly to the environment and human beings, which makes it gradually become a popular means to control bacterial wilt. Based on the isolation and screening of Pseudomonas mosselii, a rhizosphere microorganism that inhibits Ralstonia solanacearum, this study predicted the synthetic gene cluster of its antibacterial products through reverse genetics and bioinformatics, constructed deletion and complementary mutants, and identified the role of Pseudomonas mosselini, an active substance of Pseudomonas mosselii, in the resistance to Ralstonia solanacearum, in order to determine the effect of Pseudomonas mosselii in the prevention and control of Ralstonia solanacearum and other crops.
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