青枯病是一种由青枯劳尔氏菌（Ralstonia solanacearu

Ralstonia solanacearum is a highly destructive bacterial soil-borne vascular disease caused by Ralstonia solanacearum. This fungus has strong habituation and can survive in the soil and on non-host plants for a long time. At the same time, R. solanacearum has genetic diversity and is a compound species. The fungus has obvious physiological differentiation and high variability in the process of co-evolution with the host in different geographical origins. Therefore, the control of R. solanacearum has always been a difficult problem. . At present, most of the control methods for bacterial wilt are the use of disease-resistant varieties and chemical pesticide control, but the resistance of disease-resistant varieties will gradually weaken or even lose with each generation, and chemical control will also bring serious damage to the environment and endanger human health. Biological control is a means of using organisms and their metabolites to prevent and control plant diseases. Microbial control has the characteristics of no drug resistance, environment and human friendliness, making it gradually become a popular method for the prevention and treatment of bacterial wilt. In this study, based on the isolation and screening of the rhizosphere microorganism Pseudomonas mosselii (Pseudomonas mosselii) that inhibits R. and complementing mutants to identify the role of Pseudomonas morgii active substance Pseudopyronine in resisting Ralstonia solanacearum, aiming to determine the effect of Pseudomonas morganii on controlling bacterial wilt of crops such as eggplant in production.

Bacterial wilt is a highly destructive bacterial soil borne vascular bundle disease caused by Ralstonia solanacearum. This bacterium has strong habituation and can survive in soil and non host plants for a long time. At the same time, the bacterial wilt has genetic diversity and is a composite species. This bacterium exhibits significant physiological differentiation and high variability in the process of co evolution with its host in different geographical origins, making the prevention and control of bacterial wilt a long-standing challenge. At present, the methods for controlling bacterial wilt are mostly the use of disease-resistant varieties and chemical control. However, disease-resistant varieties will gradually weaken or even lose their resistance with generations, and chemical control will also cause serious damage to the environment and endanger human health. Biological control is a means of using organisms and their metabolites to control plant disease. Among them, microbial control has the characteristics of non resistance, friendly to the environment and human beings, making it gradually become a popular means of controlling bacterial wilt. On the basis of isolating and screening the rhizosphere microorganism Pseudomonas mosselii that inhibits bacterial wilt, this study predicted the synthesis of gene cluster of its bacteriostatic products through bioinformatics through reverse genetics methods, constructed deletion and complement mutants, and identified the role of Pseudomonas mosselii's active substance Pseudomonas in resistance to bacterial wilt, The aim is to determine the effectiveness of Pseudomonas Mori in preventing and controlling bacterial wilt of crops such as nightshade in production.

Bacterial wilt is a bacterial soil-borne vascular bundle disease caused by Ralstonia solanacearum. This strain has strong habitability and can survive in soil and non-host plants for a long time. At the same time, Ralstonia solanacearum is a compound species with genetic diversity. It has obvious physiological differentiation and high variability in the process of co-evolution with its host in different geographical origins, so it has always been a difficult problem to control Ralstonia solanacearum. At present, the methods to control bacterial wilt are mostly the utilization of resistant varieties and chemical control, but the resistant varieties will gradually weaken or even lose with the disease resistance from generation to generation, and chemical control will also bring serious damage to the environment and endanger human health. Biological control is a means to control plant diseases by using organisms and their metabolites, among which microbial control has the characteristics of no drug resistance and is friendly to the environment and human beings, which makes it gradually become a popular means to control bacterial wilt. Based on the isolation and screening of Pseudomonas mosselii, a rhizosphere microorganism that inhibits Ralstonia solanacearum, this study predicted the synthetic gene cluster of its antibacterial products through reverse genetics and bioinformatics, constructed deletion and complementary mutants, and identified the role of Pseudomonas mosselini, an active substance of Pseudomonas mosselii, in the resistance to Ralstonia solanacearum, in order to determine the effect of Pseudomonas mosselii in the prevention and control of Ralstonia solanacearum and other crops.