In addition to the spectra of inhibition, we observed some other differences between PlcA and 365 the other members of subgroup II, such as a higher isoelectric point (8.6) and a net charge of 366 +1. In fact some authors use the pI values and net charges to differentiate between circular 367 bacteriocins of subgroup I (pI~10 and positively charged) from circular bacteriocins of 368 subgroup II (pI 4 to 7 and uncharged or slightly negative) (9). According to this classification 369 system PlcA should be placed in an intermediate position between subgroups I and II. 370 However, we strongly believe that this peptide should be classified within subgroup II and 371 propose to modify the classification criteria and broaden the pI range for this subgroup to be 372 between 4 to ~9.