For enrichment broth, selective media, and differential media use an inoculating loop to transfer the inoculum of each testorganism to the plated or liquid media being tested. If a plated medium is being tested, streak the surface of plate with the loop infour directions to obtain a pattern of isolated colonies. Incubate the media, and examine the plated or liquid media for thecharacteristic growth of the inocula (See Tables 1, 2, 3, and 4).