植物氨基酸转运蛋白直接负责氨基酸的跨膜转运过程，分属于两个超家族的八个

Plant amino acid transporters are directly responsible for the transmembrane transport of amino acids, and they belong to eight subfamilies of two superfamily. The ANT subfamily members are mainly responsible for the transport of aromatic and neutral amino acids. In this paper, the phylogenetic tree, transmembrane structure and protein conserved motif characteristics of the members of the OsANT family were analyzed. The temporal and spatial expression characteristics of the family members and their response to adversity were studied, and the amino acid absorption-deficient yeast was used to analyze OsANT. The amino acid absorption properties. The results show that the four members of the OsANT family are evolutionarily closely related to the monocotyledonous plants of maize and sorghum, but farther from the dicotyledonous plants Arabidopsis and tomato; the analysis of protein conservation motif characteristics shows that the OsANT family members There are six conserved motifs in common with ANT members in other species; gene expression analysis shows that OsANT family genes are differentially expressed in different tissues and parts, and may be synergistically involved in the utilization of amino acids in rice; adversity The analysis of gene expression levels indicated that OSANT family genes may be involved in the response to various stresses, among which OsANT1 responds strongly to heat stress, OsANT2 to nitrogen nutrition level, OsANT3 to salt stress and OsANT4 to cadmium stress; Yes, the heterologous expression of OsANT gene in yeast did not show obvious amino acid absorption activity. This study provides important clues and basis for our study of the functions of OsANT family members and the mechanism of amino acid transport and distribution under adversity.

Plant amino acid transport protein is directly responsible for the trans-membrane transport process of amino acids, belonging to two super-families of eight sub-families, of which members of the ANT sub-family are mainly responsible for the transport of aromatic amino acids and neutral amino acids. In this paper, the system evolution tree, cross-membrane structure and protein conservative base sequence characteristics of OsANT family members are analyzed, the space-time expression characteristics of family members and the response to adversity are studied, and the amino acid absorption characteristics of OsANT are analyzed by using amino acid absorption defect yeast. The results showed that the four members of the OsANT family had a closer evolutionary relationship with the monolithic plant corn and sorghum, and a far closer relationship with the gemine leaf plant amoeba and tomatoes; The analysis of gene expression in adversity shows that the OSANT family gene may be involved in the response process of various adversity, among which OsANT1 is strongly responded to thermal stress, OsANT2 to nitrogen nutrition level, OsANT3 to salt stress, and OsANT4 to cadmium stress; This study provides an important clue and basis for us to study the function of OsANT family members and the mechanism of amino acid transport distribution in adversity.

Plant amino acid transporters are directly responsible for the transmembrane transport of amino acids and belong to eight subfamilies of two superfamilies. The members of ant subfamily are mainly responsible for the transport of aromatic and neutral amino acids. In this paper, the phylogenetic tree, transmembrane structure and protein conserved motifs of osant family members were analyzed. The temporal and spatial expression characteristics of osant family members and their responses to adversity were studied. The amino acid absorption characteristics of osant were analyzed by amino acid absorption deficient yeast. The results showed that four members of osant family were close to maize and sorghum in evolution, but far from Arabidopsis and tomato in dicotyledons; the analysis of protein conserved motifs showed that osant family members shared six conserved motifs with ant members in other species; gene expression analysis showed that osant family genes were expressed in different tissues and parts The analysis of gene expression under stress indicated that osant family genes may be involved in a variety of stress response processes, among which osant1 responded strongly to heat stress, osant2 to nitrogen nutrition level, osant3 to salt stress and osant4 to cadmium stress The heterologous expression in yeast did not show obvious amino acid absorption activity. This study provides important clues and basis for us to study the function of osant family members and the mechanism of amino acid transport and distribution under stress.<br>