近年来由于铜及含铜化合物被广泛应用于杀菌剂、化学杀虫剂及畜牧食品添加剂

近年来由于铜及含铜化合物被广泛应用于杀菌剂、化学杀虫剂及畜牧食品添加剂等，致使土壤铜含量不断增加，对土壤微生物造成不良影响，引起土壤生态系统失衡和土壤质量下降。棘孢木霉Ts93铜相关基因Tsctr3基因是酵母中用于调控铜进入胞内的跨膜蛋白。本研究通过生物信息学分析，从棘孢木霉Ts93全基因组中筛选铜相关跨膜通道蛋白及相关基因。通过基因敲除研究该基因调节木霉属细胞内铜转运的生理功能。获得一个跨膜通道蛋白Tsctr3，该基因mRNA大小为660 bp，编码219 Kda的蛋白质。通过ATMT技术获得敲除突变株ΔTsctr3，为后续Tsctr3功能研究奠定基础，同时研究木霉菌铜耐受及代谢相关机制，对提高防病效果、改善土壤生态环境等具有重要意义。<br><br>关键词：棘孢木霉；铜相关；跨膜蛋白

近年来由于铜及含铜化合物被广泛应用于杀菌剂、化学杀虫剂及畜牧食品添加剂等，致使土壤铜含量不断增加，对土壤微生物造成不良影响，引起土壤生态系统失衡和土壤质量下降。 棘孢木霉Ts93铜相关基因Tsctr3基因是酵母中用于调控铜进入胞内的跨膜蛋白。 本研究通过生物信息学分析，从棘孢木霉Ts93全基因组中筛选铜相关跨膜通道蛋白及相关基因。 通过基因敲除研究该基因调节木霉属细胞内铜转运的生理功能。 获得一个跨膜通道蛋白Tsctr3，该基因mRNA大小为660 bp，编码219 Kda的蛋白质。 通过ATMT技术获得敲除突变株ΔTsctr3，为后续Tsctr3功能研究奠定基础，同时研究木霉菌铜耐受及代谢相关机制，对提高防病效果、改善土壤生态环境等具有重要意义。<br><br>关键词：棘孢木霉；铜相关；跨膜蛋白

In recent years, copper and copper containing compounds have been widely used in fungicides, chemical pesticides and animal husbandry food additives, resulting in the increasing content of copper in soil, causing adverse effects on soil microorganisms, resulting in the imbalance of soil ecosystem and the decline of soil quality. Tsctr3, a copper related gene of Trichoderma echinosporum ts93, is a transmembrane protein used to regulate the entry of copper into yeast cells. In this study, we screened copper related transmembrane channel proteins and related genes from the whole genome of Trichoderma echinosporum ts93 by bioinformatics analysis. By gene knockout, the physiological function of this gene in regulating copper transport in Trichoderma cells was studied. A transmembrane channel protein tsctr3 was obtained. The mRNA of tsctr3 was 660 BP, encoding a 219 kDa protein. The knockout mutant Δ tsctr3 was obtained by ATMT technology, which laid the foundation for the follow-up study of tsctr3 function, and studied the copper tolerance and metabolism mechanism of Trichoderma, which was of great significance to improve the effect of disease prevention and soil ecological environment.<br>Key words: Trichoderma echinosporum; copper related; transmembrane proteins<br>